Antibiogram patterns of primary isolates of staphylococcus aureus in relation to enzymatic activity and phage type

The genus Staphylococcus is a member of the family Micrococcaceae which includes three other general Micrococcus, Planococcus and Aerococcus. The four which have the same morphology and gram reaction may be distinguished from each other on the basis of arrangement, the utilization of glucose, the presence or abscence of cytochromes, oxygen requirements, motility and the G + C content of DNA. (Table I), The genus Staphylococcus includes three species; S. aureus, S. epidermidis and S. saprophyticus. These may be distinguished from each other on the basis of coagulase production, mannitol utilization, production of alpha toxin, presence of heat resistant endonucleases, requirement of biotin for growth, sensitivity to novobiocin and cell wall composition (Table II). In the early studies involving phages types and antibiotic susceptibilities, dilution methods (MIC) were used to determine susceptibility. With the adoption of the Kirby-Bauer technique, its wide clinical use, and the dynamic changes occurring in the staphylococci with respect to antibiotic susceptibility and phage patterns. Reexamination of these relationships becomes important and essential. Since no studies of this kind have ever been attempted on staphylococci from this community, it was decided to investigate the problem with respect to enzymatic activity, antibiogram and phage type and compare the findings with those from other geographic locations

Who pays business rates?

Non-domestic rates are a tax that is formally levied on the occupiers of nondomestic property in the United Kingdom. This does not imply that it is only the occupiers of business and other non-domestic property who are made worse off by the imposition of ‘business rates’. Some or all of the effective burden of nondomestic rates may be shifted backwards from the occupiers of business property to the owners of business property. This occurs if the rents that property owners can charge their tenants are reduced by the imposition of business rates. In this case, the total cost of occupying a business property (i.e. rent plus rates) is increased by less than the full amount of the non-domestic rates paid by occupiers, and part of the burden of business rates is borne by property owners in the form of lower rental income than they would otherwise have received. The effective incidence of non-domestic rates is then said to fall partly on property owners, and only partly on occupiers.

The percutaneous absorption of soman in a damaged skin porcine model and the evaluation of WoundStat™ as a topical decontaminant

PURPOSE: The aim of this study was to evaluate a candidate haemostat (WoundStat™), down-selected from previous in vitro studies, for efficacy as a potential skin decontaminant against the chemical warfare agent pinacoyl methylfluorophosphonate (Soman, GD) using an in vivo pig model. MATERIALS AND METHODS: An area of approximately 3 cm2 was dermatomed from the dorsal ear skin to a nominal depth of 100 µm. A discrete droplet of 14C-GD (300 µg kg-1) was applied directly onto the surface of the damaged skin at the centre of the dosing site. Animals assigned to the treatment group were given a 2 g application of WoundStat™ 30 s after GD challenge. The decontamination efficacy of WoundStat™ against GD was measured by the direct quantification of the distribution of 14C-GD, as well as routine determination of whole blood cholinesterase and physiological measurements. RESULTS: WoundStat™ sequestered approximately 70% of the applied 14C-GD. Internal radiolabel recovery from treated animals was approximately 1% of the initially applied dose. Whole blood cholinesterase levels decreased to less than 10% of the original value by 15 min post WoundStat™ treatment and gradually decreased until the onset of apnoea or until euthanasia. All treated animals showed signs of GD intoxication that could be grouped into early (mastication, fasciculations and tremor), intermediate (miosis, salivation and nasal secretions) and late onset (lacrimation, body spasm and apnoea) effects. Two of the six WoundStat™ treated animals survived the study duration. CONCLUSIONS: The current study has shown that the use of WoundStat™ as a decontaminant on damaged pig ear skin was unable to fully protect against GD toxicity. Importantly, the findings indicate that the use of WoundStat™ in GD contaminated wounds would not exacerbate GD toxicity. These data suggest that absorbent haemostatic products may offer some limited functionality as wound decontaminants.Peer reviewedFinal Accepted Versio

Mechanical weathering in cold regions with special emphasis on the Antarctic environment and the freeze-thaw mechanism in particular.

Thesis (Ph.D.)-University of Natal, Pietermaritzburg, 2003.Consideration of almost any geomorphology textbook will show the fundamental argument that in cold environments mechanical weathering processes, usually freeze-thaw, will predominate and that chemical weathering will be temperature-limited, often to the point of non occurrence. These basic concepts have underpinned geomorphology for over a century and are the basis for the development of many landforms in periglacial regions. With the introduction of data loggers so field data became more readily available but, sadly, those data were not of a quality to other than justify the existent assumptions and thus did little more than reinforce, rather than test, the nature of our understanding of cold region weathering. Factors such as rock properties were dealt with to a limited extent but rock moisture was all but ignored, despite its centrality to most weathering processes. Here the results of field studies into weathering in cold regions, coupled with laboratory experiments based on the field data, are presented. An attempt is made to overcome the shortcomings of earlier studies. Temperature, moisture and rock properties have all been considered. Processes were not assumed but rather the data were used to evaluate what processes were operative. The results, both in terms of weathering process understanding per se and of its application to landform development, significantly challenge our longheld perceptions. Information is presented that shows that it is not temperature, but rather water, that is the limiting factor in cold region weathering. Indeed, in the absence of water, many cold environments have attributes akin to a hot desert. The relevance of this is that weathering processes other than freeze-thaw may play a significant role and that in the presence of water chemical weathering can play a far greater role than hitherto thought. Overall, the whole concept of zonality with respect to weathering is questioned. Finally, the attributes of weathering are put within the context of landform development and questions raised regarding the origin of some forms and of their palaeoenvironmental significance. Attributes of periglacial, glacial and zoogeomorphic processes and landforms in present and past cold environments are also presented

Do low-carbohydrate diets increase energy expenditure?

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Winter Wheat Test Results for South Dakota, 2002

The winter wheat variety recommendations for 2003 are listed in table 1. Comments: The dominant issue facing South Dakota agriculture in the 2002 crop season was the lack of moisture in the fall of 2001 and the winter and spring of 2002. Limited moisture led to a large deficit in subsoil moisture in many cropping regions of the state. This in turn resulted in many acres of winter wheat and other small grains being harvested for hay. The average winter wheat yield in the South Dakota Crop Performance Testing (CPT) Program was 39 bu/A for year 2002 and 48 bu/A for the 3-year period (2000-2002). Compared to 2001, this was a drop of 7 bu/A in both the 1- and 3-year CPT yield averages

Snowmass 2021 Underground Facilities & Infrastructure Frontier Report

The decade since Snowmass 2013 has seen extraordinary progress of high energy physics research performed--or planned for--at underground facilities. Drs. T. Kajita and A.B. McDonald were awarded the 2015 Nobel Prize in Physics for the discovery of neutrino oscillation, which show that neutrinos have mass. The U.S. has embarked on the development of the world-class LBNF/DUNE science program to investigate neutrino properties. The Generation 2 dark matter program is advancing to full data collection in the coming 5 years, a Dark Matter New Initiatives program has begun, and the U.S. dark matter community is looking toward a Generation 3 program of large-scale dark matter direct detection searches. The Sanford Underground Research Facility has become a focal point for U.S. underground facilities and infrastructure investment. The status since the 2013 Snowmass process as well as the outcome from the 2014 P5 program of recommendations is reviewed. These are then evaluated based on the activities and discussions of the Snowmass 2021 process resulting in conclusions looking forward to the coming decade of high energy physics research performed in underground facilities.Comment: Snowmass 2021 Underground Facilities & Infrastructure Frontier Repor

Using morphospaces to understand tafoni development

Tafoni research has tended to focus on issues around definition and differences rather than trying to develop general concepts for understanding the nature of tafoni. This paper uses the concepts of fitness landscapes and morphospaces to develop a standardized and dimensionless phase space within which to represent, visualize and analyze a dataset of 800 tafoni collected from Antarctica. Within this phase space it is possible to identify clustering of tafoni forms and to illustrate how tafoni development is constrained by a relational hierarchy of rock structure, processes and geometry or form.South African NRF.http://www.elsevier.com/locate/geomorph2017-05-31hb2016Geography, Geoinformatics and Meteorolog

Preface : causes of obesity, theories, conjectures and evidence

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